The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga
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0:24 - 0:27Today, I'm here to tell you
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0:27 - 0:31about the hidden treasures
of the human body, -
0:31 - 0:34the marvelous stem cells.
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0:35 - 0:39Humans are the crowning glory
of the nature, -
0:39 - 0:41and during Renaissance times
it was thought -
0:41 - 0:44that the architecture of the human body
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0:44 - 0:47represents the architecture
of the Universe. -
0:49 - 0:54This is a picture of the Vitruvian man
by Leonardo da Vinci -
0:54 - 0:57showing the geometry of the human body.
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0:58 - 1:03Renaissance period was the beginning
of the modern medicine. -
1:03 - 1:06The anatomy of the human body
was discovered, -
1:06 - 1:09and by the invention of the microscope
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1:10 - 1:14the body was studied
at the cellular level, -
1:14 - 1:17and it was found that the cell is
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1:17 - 1:20the smallest building block of the body.
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1:20 - 1:26And we are composed of 50 trillion cells.
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1:26 - 1:29A trillion is a number with twelve zeroes.
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1:30 - 1:36So how big would be
the house of trillion zeroes? -
1:36 - 1:40To imagine a house
of trillion building blocks. -
1:41 - 1:47And in fact, the Great Wall of China
is built of 4 billion building blocks. -
1:48 - 1:54And we, as human beings,
are 10 thousand times more complicated -
1:54 - 1:56than the Great Wall of China!
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1:56 - 2:01It took 2,000 years
to build the Great Wall of China -
2:01 - 2:05and perhaps you'll wonder
how long it takes to build a human being? -
2:05 - 2:10In an average, it takes
7 to 10 minutes for mom and dad -
2:10 - 2:13to put two magic building blocks together,
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2:14 - 2:17to lay the fundaments of a new life,
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2:17 - 2:23and the rest of the body building
is done by the magic stem cells. -
2:24 - 2:29I was trying to imagine
what would my life look like -
2:29 - 2:31if I lived in Renaissance times.
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2:33 - 2:37Despite of the great progress
in science and culture, -
2:37 - 2:39women were not allowed to study.
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2:41 - 2:46So luckily for me,
I live in the 21st century in Latvia, -
2:46 - 2:51where society is familiar
with gender mainstreaming ideas, -
2:51 - 2:54and I am a researcher,
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2:54 - 2:59so I chose to wear a lab coat
instead of that gorgeous dress. -
3:01 - 3:06I came to the research lab
when I was a third year biology student, -
3:06 - 3:10and I was fascinated
by the friendly atmosphere in the lab. -
3:11 - 3:15Respectable scientists were sequencing DNA
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3:15 - 3:18and during the breaks, they boiled tea
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3:18 - 3:20and smoked in the fume hood.
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3:20 - 3:24"Wow," I thought,
"this could be my dream job!" -
3:24 - 3:31So now, I have 19 years of experience
being a researcher -
3:31 - 3:36and during the past 7 years
I've been studying adult stem cells. -
3:37 - 3:42I'm very of excited
about the stem cell potential, -
3:42 - 3:44and I think that today we live
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3:44 - 3:48in the golden era
of stem cell discoveries. -
3:49 - 3:53Every part of our body
has some capacity to renew -
3:53 - 3:56due to these amazing stem cells.
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3:57 - 3:59I want to understand
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3:59 - 4:04the regeneration process
that occurs naturally -
4:04 - 4:09to find out ways
how to use stem cells to treat diseases. -
4:11 - 4:14How I got interested in cell biology?
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4:14 - 4:18One day I saw a picture
in my high school biology book -
4:18 - 4:20that looked something like this.
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4:20 - 4:23I tried to reproduce it, and it described
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4:23 - 4:26that cell consists of a membrane,
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4:26 - 4:27and there is a Golgi complex,
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4:27 - 4:30and mitochondria, and lysosomes
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4:30 - 4:34and there is a nucleus,
the director of the cell, -
4:34 - 4:38so my vivid imagination
pictured a large desk -
4:38 - 4:39in the middle of the cell
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4:39 - 4:42with a strict director giving commands.
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4:42 - 4:45When I learned to use the microscope,
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4:45 - 4:48I never saw a director inside the cell,
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4:48 - 4:53instead I learned,
that cell is a very complex structure -
4:53 - 4:57and the nucleus is rather a hard disk
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4:57 - 5:00that stores genetic programs.
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5:01 - 5:06And cells communicate
with each other by sending signals, -
5:06 - 5:10and these signals are
biological and chemical molecules. -
5:11 - 5:15The signals are transmitted
then to the nucleus, -
5:15 - 5:18and the genetic program is starting.
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5:18 - 5:24So once we know the signals
to make the desired cell type, -
5:24 - 5:28we can grow cells in a Petri dish
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5:28 - 5:33and then transplant them
into the patient and treat the disease. -
5:35 - 5:39Cell experiments are performed
in a cell culture laboratory -
5:39 - 5:46which is equipped with such equipment
as sterile biosafety cabinets, -
5:46 - 5:50incubators, microscopes,
and different chemicals. -
5:51 - 5:54And cells are grown
in special plastic bottles, -
5:54 - 5:56in liquid cell-culture medium.
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5:56 - 6:00So during the experiment,
we add a mixture of growth factors, -
6:00 - 6:06and then we observe changes
in cell shape and protein expression. -
6:06 - 6:09And in this image,
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6:09 - 6:12I wish to show you one experiment
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6:12 - 6:17that I managed to prove
that adult stem cells are similar -
6:17 - 6:20to embryonic stem cells
in their potential. -
6:21 - 6:24When I tried to publish my observation,
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6:24 - 6:27my paper was rejected three times,
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6:27 - 6:30but I didn't give up,
and now this paper is published -
6:30 - 6:35and cited 115 times
by other stem cell scientists. -
6:37 - 6:42Here is a classical experiment
to prove that you work with stem cells. -
6:42 - 6:46So to prove that these are
stem cells you have to show -
6:46 - 6:50that they're able to differentiate
into 3 distinct cell types. -
6:51 - 6:55So I added 3 different mixtures
of growth factors, -
6:55 - 7:01and as a result after 3 weeks
of experiment, I got fat cells. -
7:01 - 7:06And I can say that these are fat cells,
because I can stain oil droplets in red. -
7:07 - 7:10Then I get bone cells,
and I can say that these are bone cells -
7:10 - 7:14because I see calcium deposits
stained in orange. -
7:14 - 7:19And then the cartilage cells
that I can stain in blue. -
7:21 - 7:25Well, experiments take a long time
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7:25 - 7:28and to get these results,
it took me half a year. -
7:29 - 7:34So I wondered what takes so long
to get results in research. -
7:34 - 7:37And now, imagine that you want to prepare
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7:37 - 7:42some very complicated dish,
a very complicated recipe, -
7:42 - 7:44something like homemade mayonnaise.
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7:45 - 7:48So first, you have to get the recipe,
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7:48 - 7:50then the right ingredients,
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7:50 - 7:52and then you have to know
the special technique -
7:52 - 7:54how to mix it together.
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7:54 - 7:57And I tried twice, and I failed.
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7:57 - 8:00Well, the same happens in experiments.
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8:00 - 8:06It's the mixture of right ingredients
and a technique that makes it work. -
8:08 - 8:11And here I'm showing you skin stem cells.
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8:11 - 8:14It is the fluorescent microscopy image.
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8:14 - 8:18I use special dyes to color cells,
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8:18 - 8:21so you see the director,
the nucleus, stained in blue, -
8:22 - 8:27and the mitochondria, the power plant
of the cell, is stained in red, -
8:27 - 8:31and the cytoskeleton,
that holds the cell together -
8:31 - 8:35and gives its shape, is stained in green.
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8:36 - 8:39I can spend hours
looking into the microscope. -
8:39 - 8:42Its like seeing a different world.
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8:42 - 8:47And I think these cells look like jewels.
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8:47 - 8:52Indeed, stem cells
are our inner treasures. -
8:54 - 8:58Here is another experiment where I use
the mixture of nerve growth factors -
8:58 - 9:02to make skin stem cells
to become nerve cells. -
9:04 - 9:08It took 2 years of work
for my student Vadims -
9:08 - 9:12to establish the right procedure.
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9:12 - 9:16And now we have
a model system to find a drug -
9:16 - 9:20that would stimulate
nerve growth after trauma. -
9:21 - 9:23Make a wild guess,
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9:23 - 9:26how long it will take
for me to find this drug -
9:26 - 9:29if it took 2 years to get to this picture?
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9:31 - 9:34Breaking news fascinate
humans nearly every day. -
9:34 - 9:38We hear about restored vision,
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9:38 - 9:41about improved heart functions,
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9:41 - 9:44rebuilt urinary bladders, rebuilt trachea.
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9:45 - 9:48A lot of studies have been done
in a mouse model. -
9:49 - 9:54By the way, during my PhD studies,
I worked with thousands of mice. -
9:55 - 9:59And I really worked very hard,
and when I finished my PhD, I thought: -
9:59 - 10:02"That's it, no more mouse work!"
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10:03 - 10:06Then I got married,
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10:07 - 10:12and now I study human adult stem cells.
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10:13 - 10:17For thousands of years, humans
have been dreaming to reach the stars, -
10:17 - 10:20and yet, we have come
as far as to the Moon, -
10:21 - 10:25and for thousands of years,
people have been dreaming -
10:25 - 10:29to find the elixir of life,
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10:29 - 10:33and we are taking the first steps
in undertaking -
10:33 - 10:37how stem cells work
to regenerate the body. -
10:39 - 10:42There is enough evidence now collected
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10:42 - 10:46that stem cells from the patient
or from the donor -
10:46 - 10:48really can cure the disease.
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10:49 - 10:55There is success in clinical trials
to treat such diseases -
10:55 - 10:58such as retinal degeneration,
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10:58 - 11:01to improve heart functions
after a heart attack, -
11:01 - 11:04to stop transplant rejection,
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11:04 - 11:07to renew cartilage,
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11:07 - 11:09to heal skin lesions,
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11:09 - 11:12and to treat blood cancer,
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11:12 - 11:15autoimmune diseases like Crohn's disease,
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11:15 - 11:18and immune system's deficiencies.
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11:19 - 11:23The road is long
from stem cell research to the clinics. -
11:25 - 11:28There are significant safety standards
that must be met -
11:28 - 11:31to say that the treatment
will be safe for the patient. -
11:32 - 11:36And the main safety issues
for stem cells are -
11:36 - 11:40the potential tumorigenicity
and immunogenicity risks. -
11:43 - 11:47So I see a great similarity
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11:47 - 11:51between the Renaissance era
and the stem cell era. -
11:53 - 11:57Both increased understanding
about the human body -
11:57 - 12:00with little effect on healthcare.
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12:01 - 12:03And to increase the effect on healthcare,
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12:03 - 12:06more specialists are needed
in biotechnology, -
12:06 - 12:09bio informatics, bioengineers, healthcare
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12:09 - 12:12just to make the infrastructure
in the field. -
12:13 - 12:18The university is the incubator
of knowledge generation. -
12:19 - 12:23And I use this image
-- Earth at night -- to illustrate -
12:23 - 12:26that in each of the major light spots
there is a university. -
12:27 - 12:30And in each of the universities
there is a cell culture lab -
12:30 - 12:35and a stem cell scientist
working on discoveries -
12:35 - 12:37about stem cell use to treat diseases.
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12:37 - 12:40I think there's a great
potential in this field. -
12:43 - 12:46It takes a long time
to educate a biotechnologist, -
12:46 - 12:51so one learns how to use
advanced research equipment, -
12:51 - 12:54how to plan experiments,
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12:54 - 12:56and how to interpret the data.
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12:58 - 13:03In coming years, there will be
major advancements in stem cell use -
13:03 - 13:09in bioimplants, drug screening,
and stem cells will be uploaded -
13:09 - 13:12with anti-cancer drugs like Trojan horses
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13:12 - 13:15to reach the cancer and to destroy it.
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13:18 - 13:2323 years ago, a Nobel prize was awarded
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13:23 - 13:27for the discovery
of bone marrow transplantation. -
13:28 - 13:31That marked the beginning
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13:31 - 13:34of the golden era
of regenerative medicine. -
13:35 - 13:39I believe that stem cell based therapies
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13:39 - 13:46will become the golden standard of
healthcare for my children's generation. -
13:48 - 13:50Thank you.
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13:50 - 13:51(Applause)
- Title:
- The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga
- Description:
-
This talk was given at a local TEDx event, produced independently of the TED Conferences.
In her speech Una explains that adult stem cells are our body's natural resource that renews the body lifelong. Stem cell research helps to find out ways how to use stem cells to cure diseases like heart attack, diabetes, lost vision and autoimmune diseases. Adult stem cells are the medicines of tomorrow that will improve the quality of life for many people yet they are not the panacea for all diseases.
- Video Language:
- English
- Team:
- closed TED
- Project:
- TEDxTalks
- Duration:
- 14:00
Denise RQ edited English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Denise RQ edited English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Denise RQ approved English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Denise RQ accepted English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Denise RQ edited English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Denise RQ edited English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Denise RQ edited English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga | ||
Kristaps edited English subtitles for The golden era of stem cell discoveries | Una Riekstiņa | TEDxRiga |