[Script Info]
Title: 
[Events]
Format: Layer, Start, End, Style, Name, MarginL, MarginR, MarginV, Effect, Text
Dialogue: 0,0:00:09.00,0:00:13.00,Default,,0000,0000,0000,,(English captions by Andrea Matsumoto, University of Michigan.)
Dialogue: 0,0:00:15.00,0:00:20.00,Default,,0000,0000,0000,,These are the items we require in the stool\Nconcentration technique.
Dialogue: 0,0:00:20.00,0:00:27.00,Default,,0000,0000,0000,,We need the stool sample, the fine mesh of\Npore size three fifty to four fifteen micrometer,
Dialogue: 0,0:00:27.00,0:00:43.00,Default,,0000,0000,0000,,the fifteen mL centrifuge tube, a glass applicator\Nfor emulsifying the stool sample, the beaker
Dialogue: 0,0:00:43.00,0:00:58.00,Default,,0000,0000,0000,,for the filtrates, the ten percent Formol-saline\Nfor mixing the stool, then the diethyl ether.
Dialogue: 0,0:00:58.00,0:01:12.00,Default,,0000,0000,0000,,Add ten percent Formol-saline to the stool,\Nabout a gram of the stool sample.
Dialogue: 0,0:01:12.00,0:01:21.00,Default,,0000,0000,0000,,Emulsify the stool sample with the applicator.
Dialogue: 0,0:01:21.00,0:01:31.00,Default,,0000,0000,0000,,Emulsify it very well to get a smooth substance.
Dialogue: 0,0:01:31.00,0:01:53.00,Default,,0000,0000,0000,,Sift the stool sample through the fine mesh\Ninto the beaker.
Dialogue: 0,0:01:53.00,0:01:57.00,Default,,0000,0000,0000,,Discard the stool sample.
Dialogue: 0,0:01:57.00,0:02:03.00,Default,,0000,0000,0000,,The debris is discarded under the tap water\Nbecause it has been disinfected already with
Dialogue: 0,0:02:03.00,0:02:07.00,Default,,0000,0000,0000,,the Formol.
Dialogue: 0,0:02:07.00,0:02:17.00,Default,,0000,0000,0000,,Transfer seven mils of stool filtrate into\Nthe fifteen mil centrifuge tube.
Dialogue: 0,0:02:17.00,0:02:30.00,Default,,0000,0000,0000,,Rinse whatever is left in the beaker into\Nthe centrifuge tube.
Dialogue: 0,0:02:30.00,0:02:40.00,Default,,0000,0000,0000,,Add three mils of the diethyl ether to the\Nseven mil of the stool filtrate in the fifteen
Dialogue: 0,0:02:40.00,0:02:47.00,Default,,0000,0000,0000,,mil centrifuge tube making it to a total of\Nten mL.
Dialogue: 0,0:02:47.00,0:02:53.00,Default,,0000,0000,0000,,And we will see two layers: the ether layer\Nand then the Formol-saline layer.
Dialogue: 0,0:02:53.00,0:03:06.00,Default,,0000,0000,0000,,Cover the centrifuge tube with a lid and mix\Nthe two layers very well.
Dialogue: 0,0:03:06.00,0:03:12.00,Default,,0000,0000,0000,,And thus the ether is to dissolve in the fat,\Nwhich is present in the stool sample, to release
Dialogue: 0,0:03:12.00,0:03:16.00,Default,,0000,0000,0000,,the parasites.
Dialogue: 0,0:03:16.00,0:03:30.00,Default,,0000,0000,0000,,Put it in the centrifuge tube balancing it.
Dialogue: 0,0:03:30.00,0:03:40.00,Default,,0000,0000,0000,,Centrifuge at thousand five hundred (1500) RPM for\Ntwo to five minutes.
Dialogue: 0,0:03:40.00,0:03:45.00,Default,,0000,0000,0000,,Okay take out the centrifuge tube and you\Nwill see four layers.
Dialogue: 0,0:03:45.00,0:03:51.00,Default,,0000,0000,0000,,The first layer is the ether layer, the debris\Nlayer, which is the insoluble pad, and then
Dialogue: 0,0:03:51.00,0:04:04.00,Default,,0000,0000,0000,,Formol-saline layer, and then the sediment\Nwhich contains the parasite.
Dialogue: 0,0:04:04.00,0:04:17.00,Default,,0000,0000,0000,,Break through the debris layer and discard\Nthe first three layers under the tap water,
Dialogue: 0,0:04:17.00,0:04:22.00,Default,,0000,0000,0000,,which have already been treated with Formol.
Dialogue: 0,0:04:22.00,0:04:29.00,Default,,0000,0000,0000,,So the sediment will contain the parasites\Nof the egg or the larvae and then cysts were
Dialogue: 0,0:04:29.00,0:04:36.00,Default,,0000,0000,0000,,suspended with part of the Formol-saline or\Nsaline.
Dialogue: 0,0:04:36.00,0:04:43.00,Default,,0000,0000,0000,,Use a disposable pipet to transmit it very\Nwell and transfer a drop onto the microscope
Dialogue: 0,0:04:43.00,0:04:45.00,Default,,0000,0000,0000,,slide.
Dialogue: 0,0:04:45.00,0:04:57.00,Default,,0000,0000,0000,,Cover the drop of smear with the cover slip\Nand examine it under the microscope using
Dialogue: 0,0:04:57.00,0:05:01.00,Default,,0000,0000,0000,,the times ten objective (10X).
Dialogue: 0,0:05:01.00,0:05:05.00,Default,,0000,0000,0000,,Low light allows better to see the parasite\Nof the egg, the cyst, and the larvae.